Musculoskeletal tissue regeneration occurs within the context of a three-dimensional microenvironment that relies upon dynamic interplay between biochemical and biophysical cues. The cellular and molecular impact of this interplay on muscle stem cell fate is not well understood. To gain a better understanding of the combinatorial inputs required to achieve tissue regeneration, we use two approaches: (1) engineered culture models to reconstruct parameters in vitro and (2) in vivo manipulation of individual parameters. These studies will inform future efforts aimed at developing engineered replacement musculoskeletal tissue for regenerative medicine applications.
Bakooshli MA, Lippman ES, Mulcahy B, Iyer N, Nguyen CT, Tung K, Stewart BA, van den Dorpel H, Fuehrmann T, Shoichet MS, Bigot A, Pegoraro E, Ahn H, Ginsberg H, Zhen M, Ashton RS, and Gilbert PM. (2018). A three-dimensional culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction and disease modeling. BioRxiv, https://doi.org/10.1101/275545.
Davoudi S, Chin C-Y, Cooke MC, Tam RY, Shoichet MS, and Gilbert PM. (2018) Muscle stem cell intramuscular delivery within hyaluronin methylcellulose improves engraftment efficiency and dispersion. Biomaterials, 173: 34-46.